Molecular Cloning, Nucleotide Sequence, and Tissue Distribution of Malonyl-CoA Decarboxylase
نویسندگان
چکیده
منابع مشابه
Molecular cloning, nucleotide sequence, and tissue distribution of malonyl-CoA decarboxylase.
Malonyl-CoA decarboxylase was purified from goose uropygial gland, reduced, carboxymethylated, and digested with trypsin. Several peptides were purified by high performance liquid chromatography and their amino acid sequences determined. Oligonucleotide probes were prepared based on their amino acid sequences. Size-selected RNA from the goose uropygial gland was used to construct cDNA libraries...
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background the malonyl-coa decarboxylase (mcd, ec.4.1.1.9) enzyme regulates malonyl-coa levels. the effect of aerial parts extracts of urtica dioica (ud) on mcd is poorly understood. objectives the present experiment was undertaken to evaluate the effect of ud aerial parts extracts on mcd level. materials and methods in this experimental study, two groups of rats were used: normal and hyperglyc...
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Nicolas VOILLEY*, Raphae$ l RODUIT*, Raffaela VICARETTI*, Christophe BONNY†, Ge! rard WAEBER†, Jason R. B. DYCK‡, Gary D. LOPASCHUK‡ and Marc PRENTKI* *Molecular Nutrition Unit, Department of Nutrition, University of Montreal and the CR Centre Hospitalier de l’Universite! de Montre! al and Institut du Cancer, 1560 Sherbrooke Est, Montreal, Quebec H2L 4M1, Canada, †Department of Internal Medicin...
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Malonyl-CoA decarboxylase (MCD) is an enzyme involved in the decarboxylation of malonyl-CoA to acetyl-CoA. In order to explore the hypothesis that the changing plant materials’ MCD activity level can serve as therapy to diabetics, the effect of Teucrium polium compounds was studied in a diabetic rat model. In this experimental study, two groups of rats, a control and a diabetic group, each incl...
متن کاملRat malonyl-CoA decarboxylase; cloning, expression in E. coli and its biochemical characterization.
Malonyl-CoA decarboxylase (E.C.4.1.1.9) catalyzes the conversion of malonyl-CoA to acetyl-CoA. Although the metabolic role of this enzyme has not been fully defined, it has been reported that its deficiency is associated with mild mental retardation, seizures, hypotonia, cadiomyopathy, developmental delay, vomiting, hypoglycemia, metabolic acidosis, and malonic aciduria. Here, we isolated a cDN...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1989
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)94094-9